Physio-chemical and morphological characteristics of avian encephalomyelitis virus
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Abstract
Avian encephalomyelitis virus (AEV) was purified from infected chick embryos by a gradient centrifugation in cesium chloride. The virus had a buoyant density of 1.31 to 1.32 g/ml and a sedimentation coefficient of 148 S. The purified AEV was resistant to treatments with chloroform, acid pH or trypsin. The presence of Mg++ stabilized the virus against heat inactivation (56°C, 1 h). Electron microscopic study showed the virus to be 24 to 32 nm in diameter. The surface structure of the purified virus was not easily discernable. Nevertheless, with uranyl acetate-stained particles, Markham's rotation technique revealed that AEV has five-fold symmetry with 32 or 42 capsomers. Exact classification of AEV awaits characterization of the viral nucleic acid.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Physio-chemical and morphological characteristics of avian encephalomyelitis virus |
| Series title | Veterinary Microbiology |
| DOI | 10.1016/0378-1135(80)90013-9 |
| Volume | 5 |
| Issue | 2 |
| Year Published | 1980 |
| Language | English |
| Publisher | Elsevier |
| Publisher location | Amsterdam |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 14 p. |
| First page | 87 |
| Last page | 100 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |