A new panel of SNP markers for the individual identification of North American pumas

Journal of Fish and Wildlife Management
By: , and 

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Abstract

Pumas Puma concolor are one of the most studied terrestrial carnivores because of their widespread distribution, substantial ecological impacts, and conflicts with humans. Over the past decade, managing pumas has involved extensive efforts including the use of genetic methods. Microsatellites have been the most commonly used genetic markers; however, technical artifacts and little overlap of frequently used loci render large-scale comparison of puma genetic data across studies challenging. Therefore, a panel of genetic markers that can produce consistent genotypes across studies without the need for extensive calibrations is essential for range-wide genetic management of puma populations. Here, we describe the development of PumaPlex, a high-throughput assay to genotype 25 single nucleotide polymorphisms in pumas. We validated PumaPlex in 748 North American pumas Puma concolor couguar, and demonstrated its ability to generate reproducible genotypes and accurately identify individuals. Furthermore, in a test using fecal deoxyribonucleic acid (DNA) samples, we found that PumaPlex produced significantly more genotypes with fewer errors than 12 microsatellite loci, 8 of which are commonly used. Our results demonstrate that PumaPlex is a valuable tool for the genetic monitoring and management of North American puma populations. Given the analytical simplicity, reproducibility, and high-throughput capability of single nucleotide polymorphisms, PumaPlex provides a standard panel of markers that promotes the comparison of genotypes across studies and independent of the genotyping technology used.

 

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A new panel of SNP markers for the individual identification of North American pumas
Series title Journal of Fish and Wildlife Management
DOI 10.3996/112014-JFWM-080
Volume 7
Issue 1
Year Published 2016
Language English
Publisher U.S. Fish and Wildlife Service
Contributing office(s) Coop Res Unit Seattle
Description 15 p.
First page 13
Last page 27
Online Only (Y/N) N
Additional Online Files (Y/N) N