Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunization
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Abstract
The DNA vaccine, pIHNVw-G, contains the gene for the glycoprotein (G) of the rhabdovirus infectious hematopoietic necrosis virus (IHNV), a major pathogen of salmon and trout. The relative efficacy of various routes of immunisation with pIHNVw-G was evaluated using 1.8 g rainbow trout fry vaccinated via intramuscular injection, scarification of the skin, intraperitoneal injection, intrabuccal administration, cutaneous particle bombardment using a gene gun, or immersion in water containing DNA vaccine-coated beads. Twenty-seven days after vaccination neutralising antibody titres were determined, and 2 days later groups of vaccinated and control unvaccinated fish were subjected to an IHNV immersion challenge. Results of the virus challenge showed that the intramuscular injection and the gene gun immunisation induced protective immunity in fry, while intraperitoneal injection provided partial protection. Neutralising antibodies were not detected in sera of vaccinated fish regardless of the route of immunisation used, suggesting that cell mediated immunity may be at least partially responsible for the observed protection.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunization |
| Series title | Fish and Shellfish Immunology |
| DOI | 10.1006/fsim.2000.0286 |
| Volume | 10 |
| Issue | 8 |
| Year Published | 2000 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 13 p. |
| First page | 711 |
| Last page | 723 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |