Novel molecular markers differentiate Oncorhynchus mykiss (rainbow trout and steelhead) and the O. clarki (cutthroat trout) subspecies

Molecular Ecology Notes
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Abstract

A suite of 26 PCR-based markers was developed that differentiates rainbow (Oncorhynchus mykiss) and coastal cutthroat trout (O. clarki clarki). The markers also differentiated rainbow from other cutthroat trout subspecies (O. clarki), and several of the markers differentiated between cutthroat trout subspecies. This system has numerous positive attributes, including: nonlethal sampling, high species-specificity and products that are easily identified and scored using agarose gel electrophoresis. The methodology described for developing the markers can be applied to virtually any system in which numerous markers are desired for identifying or differentiating species or subspecies.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Novel molecular markers differentiate Oncorhynchus mykiss (rainbow trout and steelhead) and the O. clarki (cutthroat trout) subspecies
Series title Molecular Ecology Notes
DOI 10.1046/j.1471-8286.2002.00181.x
Volume 2
Issue 3
Year Published 2002
Language English
Publisher Wiley
Contributing office(s) Western Fisheries Research Center
Description 6 p.
First page 197
Last page 202
Online Only (Y/N) N
Additional Online Files (Y/N) N