Molecular evidence of undescribed Ceratonova sp. (Cnidaria: Myxosporea) in the freshwater polychaete, Manayunkia speciosa, from western Lake Erie

Journal of Invertebrate Pathology
By: , and 

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Abstract

We used PCR to screen pooled individuals of Manayunkia speciosa from western Lake Erie, Michigan, USA for myxosporean parasites. Amplicons from positive PCRs were sequenced and showed a Ceratonova species in an estimated 1.1% (95% CI = 0.46%, 1.8%) of Mspeciosa individuals. We sequenced 18S, ITS1, 5.8S, ITS2 and most of the 28S rDNA regions of this Ceratonova sp., and part of the protein-coding EF2 gene. Phylogenetic analyses of ribosomal and EF2 sequences showed the Lake Erie Ceratonova sp. is most similar to, but genetically distinct from, Ceratonova shasta. Marked interspecific polymorphism in all genes examined, including the ITS barcoding genes, along with geographic location suggests this is an undescribed Ceratonova species. COI sequences showed Mspeciosa individuals in Michigan and California are the same species. These findings represent a third parasite in the genus Ceratonovapotentially hosted by Mspeciosa.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Molecular evidence of undescribed Ceratonova sp. (Cnidaria: Myxosporea) in the freshwater polychaete, Manayunkia speciosa, from western Lake Erie
Series title Journal of Invertebrate Pathology
DOI 10.1016/j.jip.2016.05.001
Volume 137
Year Published 2016
Language English
Publisher Society for Invertebrate Pathology
Publisher location New York, NY
Contributing office(s) Great Lakes Science Center
Description 5 p.
First page 49
Last page 53
Country United States
State Michigan
Other Geospatial Lake Erie
Online Only (Y/N) N
Additional Online Files (Y/N) N