Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus

Maureen K. Purcell; Krista M. Nichols; James R. Winton; Gael Kurath; Gary H. Thorgaard; Paul Wheeler; John D. Hansen; Russell P. Herwig; Linda K. Park

doi:10.1016/j.molimm.2005.12.005

Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus

Molecular Immunology

By: Maureen K. Purcell, Krista M. Nichols, James R. Winton, Gael Kurath, Gary H. Thorgaard, Paul Wheeler, John D. Hansen, Russell P. Herwig, and Linda K. Park

https://doi.org/10.1016/j.molimm.2005.12.005

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Abstract

The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine. Gene expression in muscle tissue (I.M. site) was evaluated using a 16,008 feature salmon cDNA microarray. Eighty different genes were significantly modulated in the vector DNA group while 910 genes were modulated in the IHNV DNA vaccinate group relative to control group. Quantitative reverse-transcriptase PCR was used to examine expression of selected immune genes at the I.M. site and in other secondary tissues. In the localized response (I.M. site), the magnitudes of gene expression changes were much greater in the vaccinate group relative to the vector DNA group for the majority of genes analyzed. At secondary systemic sites (e.g. gill, kidney and spleen), type I IFN-related genes were up-regulated in only the IHNV DNA vaccinated group. The results presented here suggest that the IHNV DNA vaccine induces up-regulation of the type I IFN system across multiple tissues, which is the functional basis of early anti-viral immunity.

Additional publication details
Publication type	Article
Publication Subtype	Journal Article
Title	Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus
Series title	Molecular Immunology
DOI	10.1016/j.molimm.2005.12.005
Volume	43
Issue	13
Year Published	2006
Language	English
Publisher	Elsevier
Contributing office(s)	Western Fisheries Research Center
Description	18 p.
First page	2089
Last page	2106
Online Only (Y/N)	N
Additional Online Files (Y/N)	N
Google Analytic Metrics	Metrics page