Some enzymatic properties of brain Acetylcholinesterase from bluegill and channel catfish
Journal of the Fisheries Research Board of Canada
By: James W. Hogan and Charles O. Knowles
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Abstract
Using a manometric technique an acetylcholinesterase (EC 3.1.1.7, acetylcholine acetyl-hydrolase) was demonstrated in brain tissue from the bluegill, Lepomis macrochirus Rafinesque, and the channel catfish, Ictalurus punctatus (Walbaum). The activities were 19 and 37 μmoles acetylcholine hydrolyzed/milligram protein per hour for the bluegill and channel catfish enzymes, respectively. The optimum substrate concentration for the hydrolysis of acetylcholine was 10 mMfor the enzymes from both species. Generally, the catfish acetylcholinesterase was somewhat more susceptible than the bluegill to the inhibitors tested; however, the bluegill enzyme was more susceptible to inhibition by malathion and malaoxon.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Some enzymatic properties of brain Acetylcholinesterase from bluegill and channel catfish |
| Series title | Journal of the Fisheries Research Board of Canada |
| DOI | 10.1139/f68-059 |
| Volume | 25 |
| Issue | 4 |
| Year Published | 1968 |
| Language | English |
| Publisher | NRC Research Press |
| Contributing office(s) | Columbia Environmental Research Center |
| Description | 9 p. |
| First page | 615 |
| Last page | 623 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |