The Carolina northern flying squirrel Glaucomys sabrinus coloratus is an endangered subspecies that is restricted to high elevation forests in the southern Appalachian Mountains. Owing to rugged terrain and nocturnal habits, the subspecies’ natural history, home range characteristics and habitat preferences are poorly known. We radio-tracked 3 female and 2 male Carolina northern flying squirrels during late winter through spring 2012 in the Pisgah National Forest, North Carolina, USA. Tracked squirrels used 13 yellow birch Betula alleghaniensis and 9 red spruce Picea rubens as diurnal dens. Ten of the yellow birch dens were in cavities, whereas the remainders were dreys. Conversely, 8 of the red spruce dens were dreys and one was in a cavity. Mean (±SE) female 95 and 50% adaptive kernel home ranges were 6.50 ± 2.19 and 0.93 ± 0.33 ha, respectively, whereas the corresponding values for males were 12.6 ± 0.9 and 1.45 ± 0.1 ha, respectively. Squirrels used red spruce stands with canopies >20 m more than expected based on availability at the landscape and home range scales. Results should be interpreted cautiously because of small sample sizes and seasonal observations; however, they provide evidence that although northern hardwoods such as yellow birch are an important den habitat component, mature red spruce-dominated habitats with complex structure provide foraging habitats and are also den habitat. Our findings support efforts to improve the structural condition of extant red spruce forests and/or increase red spruce acreage to potentially benefit Carolina northern flying squirrels.