Standardization of stain used for diagnosing erythrocytic inclusion body syndrome (EIBS)
Links
- More information: Publisher Index Page
- Download citation as: RIS | Dublin Core
Abstract
Erythrocytic inclusion body syndrome (EIBS), a viral erythrocytic necrosis (VEN)-like disease, has been observed in several areas in the Northwest. This virus disease is clinically diagnosed by microscopic examination of blood smears for intracytoplasmic erythrocytic inclusion bodies. Fish biologists involved in EIBS diagnostic work have been using several types of hematological stains. It became apparent that standardization of the staining procedure was needed. Comparative tests were conducted on blood smears and kidney imprints with the following commonly used blood stains: (1) Leishman-Giesma, (2) Pinacyanol chloride, (3) Powell 's Giemsa, (4) Harleco's Giemsa, (5) Diff Quik differential stain, (6) Wright's.
Pinacyanol chloride stain was found to be the most consistent. The following staining procedure is recommended.
| Publication type | Report |
|---|---|
| Publication Subtype | Organization Series |
| Title | Standardization of stain used for diagnosing erythrocytic inclusion body syndrome (EIBS) |
| Series title | Fish Health Newsletter |
| Volume | 15 |
| Issue | 2 |
| Year Published | 1987 |
| Language | English |
| Publisher | Fish Health Section of the American Fisheries Society |
| Description | 1 p. |
| First page | 7 |
| Google Analytic Metrics | Metrics page |