Standardization of stain used for diagnosing erythrocytic inclusion body syndrome (EIBS)

Fish Health Newsletter

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Abstract

Erythrocytic inclusion body syndrome (EIBS), a viral erythrocytic necrosis (VEN)-like disease, has been observed in several areas in the Northwest. This virus disease is clinically diagnosed by microscopic examination of blood smears for intracytoplasmic erythrocytic inclusion bodies. Fish biologists involved in EIBS diagnostic work have been using several types of hematological stains. It became apparent that standardization of the staining procedure was needed. Comparative tests were conducted on blood smears and kidney imprints with the following commonly used blood stains: (1) Leishman-Giesma, (2) Pinacyanol chloride, (3) Powell 's Giemsa, (4) Harleco's Giemsa, (5) Diff Quik differential stain, (6) Wright's.

Pinacyanol chloride stain was found to be the most consistent. The following staining procedure is recommended.

Additional publication details

Publication type Report
Publication Subtype Organization Series
Title Standardization of stain used for diagnosing erythrocytic inclusion body syndrome (EIBS)
Series title Fish Health Newsletter
Volume 15
Issue 2
Year Published 1987
Language English
Publisher Fish Health Section of the American Fisheries Society
Description 1 p.
First page 7