Evaluation of a method for quantifying eugenol concentrations in the fillet tissue from freshwater fish species

Journal of AOAC International
By: , and 

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Abstract

AQUI-S 20E® (active ingredient, eugenol; AQUI-S New Zealand Ltd, Lower Hutt, New Zealand) is being pursued for approval as an immediate-release sedative in the United States. A validated method to quantify the primary residue (the marker residue) in fillet tissue from AQUI-S 20E–exposed fish was needed. A method was evaluated for determining concentrations of the AQUI-S 20E marker residue, eugenol, in freshwater fish fillet tissue. Method accuracies from fillet tissue fortified at nominal concentrations of 0.15, 1, and 60 μg/g from six fish species ranged from 88–102%. Within-day and between-day method precisions (% CV) from the fortified tissue were ≤8.4% CV. There were no coextracted compounds from the control fillet tissue of seven fish species that interfered with eugenol analyses. Six compounds used as aquaculture drugs did not interfere with eugenol analyses. The lower limit of quantitation (LLOQ) was 0.012 μg/g. The method was robust, i.e., in most cases, minor changes to the method did not impact method performance. Eugenol was stable in acetonitrile–water (3 + 7, v/v) for at least 14 days, in fillet tissue extracts for 4 days, and in fillet tissue stored at ~ −80°C for at least 84 days.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Evaluation of a method for quantifying eugenol concentrations in the fillet tissue from freshwater fish species
Series title Journal of AOAC International
DOI 10.5740/jaoacint.15-0161
Volume 99
Issue 2
Year Published 2016
Language English
Publisher Association of Official Agricultural Chemists (AOAC)
Publisher location Arlington, VA
Contributing office(s) Upper Midwest Environmental Sciences Center
First page 558
Last page 564
Online Only (Y/N) N
Additional Online Files (Y/N) N