Evaluation of a method for quantifying eugenol concentrations in the fillet tissue from freshwater fish species
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
AQUI-S 20E® (active ingredient, eugenol; AQUI-S New Zealand Ltd, Lower Hutt, New Zealand) is being pursued for approval as an immediate-release sedative in the United States. A validated method to quantify the primary residue (the marker residue) in fillet tissue from AQUI-S 20E–exposed fish was needed. A method was evaluated for determining concentrations of the AQUI-S 20E marker residue, eugenol, in freshwater fish fillet tissue. Method accuracies from fillet tissue fortified at nominal concentrations of 0.15, 1, and 60 μg/g from six fish species ranged from 88–102%. Within-day and between-day method precisions (% CV) from the fortified tissue were ≤8.4% CV. There were no coextracted compounds from the control fillet tissue of seven fish species that interfered with eugenol analyses. Six compounds used as aquaculture drugs did not interfere with eugenol analyses. The lower limit of quantitation (LLOQ) was 0.012 μg/g. The method was robust, i.e., in most cases, minor changes to the method did not impact method performance. Eugenol was stable in acetonitrile–water (3 + 7, v/v) for at least 14 days, in fillet tissue extracts for 4 days, and in fillet tissue stored at ~ −80°C for at least 84 days.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Evaluation of a method for quantifying eugenol concentrations in the fillet tissue from freshwater fish species |
| Series title | Journal of AOAC International |
| DOI | 10.5740/jaoacint.15-0161 |
| Volume | 99 |
| Issue | 2 |
| Year Published | 2016 |
| Language | English |
| Publisher | Association of Official Agricultural Chemists (AOAC) |
| Publisher location | Arlington, VA |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| First page | 558 |
| Last page | 564 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |