Characterization of poxviruses from forest birds in Hawaii

Journal of Wildlife Diseases
By: , and 



Two strains of avian pox viruses were isolated from cutaneous lesions in Hawaiian crows (Corvus hawaiiensis) examined in 1994 and a third from a biopsy obtained in 1992 from an infected bird of the Apapane species (Himatione sanguinea) by inoculation of the chorioallantoic membranes (CAM) of developing chicken embryos. The resulting proliferative CAM lesions contained eosinophilic cytoplasmic inclusion bodies characteristic of pox virus infection. The pathogenicity of these three viruses in domestic chickens was mild as evidenced by the development of relatively minor lesions of short duration at the sites of inoculation. Their virulence in this host was similar to that of a fowlpox virus (FPV) vaccine strain and contrasted greatly with the ability of two field strains of FPV to produce extensive proliferative lesions. One of the Hawaiian crow pox virus isolates as well as the one originating from the Apapane species could be propagated in two secondary avian cell lines, QT-35 and LMH. A comparison of the restriction fragment length polymorphisms (RFLP) of the genomes of the two cell line-adapted viruses, generated by EcoRI digestion, revealed a limited degree of similarity. Moreover, neither profile was comparable to those of the two field isolates of FPV, which were almost indistinguishable from each other. Thus, based on the genetic distinctness of the two Hawaiian bird viruses, they appear to represent different strains of avipoxvirus.

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Publication type Article
Publication Subtype Journal Article
Title Characterization of poxviruses from forest birds in Hawaii
Series title Journal of Wildlife Diseases
DOI 10.7589/0090-3558-36.2.225
Volume 36
Issue 2
Year Published 2000
Language English
Publisher Wildlife Disease Association
Publisher location Lawrence, KS
Contributing office(s) Pacific Island Ecosystems Research Center
Description 6 p.
First page 225
Last page 230
Country United States
State Hawaii
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