Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR

Mycological Research
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Abstract

A collection of 39 isolates of Colletotrichum acutatum, C. fragariae and C. gloeosporioides, which cause anthracnose on strawberry, was grouped into species based on the arbitrarily primed polymerase chain reaction (ap-PCR). All isolates used had previously been identified according to classical taxonomic morphology. Ap-PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of C. acutatum, C. fragariae and two genotypes of C. gloeosporioides. Fifteen of the 18 C. acutatum isolates were very similar, although three isolates which produced a red pigment had distinctly different banding patterns. Nearly identical banding patterns were observed for all nine isolates of C. fragariae. The 12 C. gloeosporioides isolates were more diverse and two separate genotypes, Cgl-1 (six isolates) and Cgl-2 (five isolates) were distinguished by ap-PCR. An additional isolate did not conform to either the Cgl-1 or Cgl-2 genotypes. The utility of ap-PCR compared with other molecular techniques for reliable identification of Colletotrichum isolates pathogenic on strawberry is discussed.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR
Series title Mycological Research
DOI 10.1016/S0953-7562(09)80653-9
Volume 99
Issue 4
Year Published 1995
Language English
Publisher Elsevier
Contributing office(s) Western Fisheries Research Center
Description 4 p.
First page 501
Last page 504