Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR
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Abstract
A collection of 39 isolates of Colletotrichum acutatum, C. fragariae and C. gloeosporioides, which cause anthracnose on strawberry, was grouped into species based on the arbitrarily primed polymerase chain reaction (ap-PCR). All isolates used had previously been identified according to classical taxonomic morphology. Ap-PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of C. acutatum, C. fragariae and two genotypes of C. gloeosporioides. Fifteen of the 18 C. acutatum isolates were very similar, although three isolates which produced a red pigment had distinctly different banding patterns. Nearly identical banding patterns were observed for all nine isolates of C. fragariae. The 12 C. gloeosporioides isolates were more diverse and two separate genotypes, Cgl-1 (six isolates) and Cgl-2 (five isolates) were distinguished by ap-PCR. An additional isolate did not conform to either the Cgl-1 or Cgl-2 genotypes. The utility of ap-PCR compared with other molecular techniques for reliable identification of Colletotrichum isolates pathogenic on strawberry is discussed.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Differentiation of Colletotrichum species responsible for anthracnose of strawberry by arbitrarily primed PCR |
| Series title | Mycological Research |
| DOI | 10.1016/S0953-7562(09)80653-9 |
| Volume | 99 |
| Issue | 4 |
| Year Published | 1995 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 4 p. |
| First page | 501 |
| Last page | 504 |
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