Determination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmonid anaemia

Diseases of Aquatic Organisms
By: , and 

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Abstract

Plasma was collected from Atlantic salmon Salrno salar with acute infectious salmon anaemia (ISA) and used to challenge Atlantic salmon parr by intraperitoneal injection. Treatment of plasma with the lipid solvent, chloroform, showed that the etiological agent of ISA contained essential lipids, probably as a viral envelope. Some infectivity remained following treatment with freon. Injection challenges using fractions from equilibrium density gradient centrifugation of plasma from fish with acute ISA revealed a band of infectivity in the range 1.184 to 1.262 g cm-3. The band was believed to conta~n both complete ISA-virus particles and infectious particles lacking a complete envelope, nucleocapsid or genome. Density gradient centrifugation of infectious plasma for enrichment of the putative ISA virus appeared to offer a suitable method for obtaining virus-specific nucleic acid for use in the construction of cDNA libraries. 

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Determination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmonid anaemia
Series title Diseases of Aquatic Organisms
DOI 10.3354/dao015225
Volume 15
Year Published 1993
Language English
Publisher Inter-Research
Contributing office(s) Western Fisheries Research Center
Description 4 p.
First page 225
Last page 228
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