thumbnail

Identification of viral hemorrhagic septicemia virus isolated from Pacific cod Gadus macrocephalus in Prince William Sound Alaska, USA

Diseases of Aquatic Organisms
By: , and 

Links

  • The Publications Warehouse does not have links to digital versions of this publication at this time
  • Download citation as: RIS | Dublin Core

Abstract

Ulcerative slun tissues from 2 Pacific cod Gadus rnacrocephalus caught in Prince William Sound, Alaska, USA, were examined for virus by Fish Pathology staff within the F.R.E.D. Division of the Alaska Department of Fish and Game. Six days after inoculation of Epitheliorna papulosum cyprini (EPC) cells at 14"C, diffuse rounding and lifting of cells from the monolayers suggestive of cytopathlc effect became visible in the lower sample dilutions. Ultrastructural examinations of affected EPC cells showed rhabdovirus particles within cytoplasmic vacuoles and on the cell surface membranes. Virus isolates from both cod were subsequently confirmed as viral hemorrhagic septicemia virus (VHSV) by serum neutralizabon and immunoblot assay. This is the first VHSV isolated from Pacific cod, which represents a new host species for the virus. Histologically, cod skin ulcers appeared to be caused by a foreign-body-type inflammatory response to foci of protozoa resembling X cells that also had plasmodial stages. Whether the rhabdovirus was incidental to the slun lesion or played a role in its etiology remains to be determined. The possible relationship between thls virus and the recent occurrences of VHSV in anadromous salmoruds from Washington State, USA, is discussed.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Identification of viral hemorrhagic septicemia virus isolated from Pacific cod Gadus macrocephalus in Prince William Sound Alaska, USA
Series title Diseases of Aquatic Organisms
Volume 12
Year Published 1992
Language English
Publisher Inter-Research
Contributing office(s) Western Fisheries Research Center
Description 9 p.
First page 167
Last page 175
Google Analytic Metrics Metrics page
Additional metadata about this publication, not found in other parts of the page is in this table