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Demonstration of the salmonid humoral response to Renibacterium salmoninarum using a monoclonal antibody against salmonid immunoglobulin

Journal of Aquatic Animal Health

By:
https://doi.org/10.1577/1548-8667(1991)003<0254:DOTSOT>2.3.CO;2

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Abstract

The specificity of the antibody response of salmonids to Renibacterium salmoninarum antigens was demonstrated by western blotting techniques that utilized a monoclonal antibody against salmonid immunoglobulin. In this study, the specificity of the response in immunized chinook salmon Oncorhynchus tshawytschawas compared with the response in naturally infected chinook salmon and coho salmon O. kisutch, and immunized rabbits. The antibody response in immunized salmon and rabbits and the naturally infected fish was primarily against the 57–58kilodalton protein complex. In addition to recognizing these proteins in the extracellular fraction and whole-cell preparations, antibody from the immunized salmon and rabbits detected four proteins with lower molecular masses. Western blotting techniques allow identification of the specific antigens recognized and are a useful tool for comparing the immunogenicity of different R. salmoninarumpreparations. Immunofluorescent techniques with whole bacteria were less sensitive than western blotting in detecting salmonid anti-R. salmoninarumantibody.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Demonstration of the salmonid humoral response to Renibacterium salmoninarum using a monoclonal antibody against salmonid immunoglobulin
Series title:
Journal of Aquatic Animal Health
DOI:
10.1577/1548-8667(1991)003<0254:DOTSOT>2.3.CO;2
Volume:
3
Issue:
4
Year Published:
1991
Language:
English
Publisher:
Taylor & Francis
Contributing office(s):
Western Fisheries Research Center
Description:
6 p.
First page:
254
Last page:
259