Strains of infectious hematopoietic necrosis (IHN) virus may be identified by structural protein differences
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Abstract
The development of an effective vaccine to infectious hematopoietic necrosis virus (IHNV) in fish requires a knowledge of the virus serotypes in nature. At least two serotypes were found among three IHNV strains (12). Attempts in our laboratory to extend this study with additional virus strains by classical immunological techniques were unsatisfactory. Thus, we sought another method for comparing IHNV strains. We report here that different strains of IHNV can be distinguished by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the viron polypeptides. Major strain differences were noted in the molecular weights of the envelope glycoprotein, G, and the nucleocapsid protein, N.
The strains of IHNV were established from samples taken at several locations in California, Oregon, Washington, and Alaska. These strains were also characterized by growth rate at 15° and 18°C and average plaque size. Distinct differences in growth rate at the two temperatures were found for the California strains. The California strains were distinguished from the other strains by the production of minute plaques.
| Publication type | Conference Paper |
|---|---|
| Publication Subtype | Conference Paper |
| Title | Strains of infectious hematopoietic necrosis (IHN) virus may be identified by structural protein differences |
| Series title | Developments in Biological Standardization |
| ISBN | 3-8055-3471-X |
| Volume | 49 |
| Year Published | 1981 |
| Language | English |
| Publisher | S. Karger |
| Publisher location | Basel, Switzerland |
| Contributing office(s) | Alaska Science Center, Western Fisheries Research Center |
| Description | 13 p. |
| Larger Work Type | Book |
| Larger Work Subtype | Conference publication |
| Larger Work Title | Fish biologics: Serodiagnostics and vaccines (Developments in biological standardization, volume 49) |
| First page | 43 |
| Last page | 55 |
| Conference Title | International Symposium on Fish Biologics |
| Conference Location | Leetown, WV |
| Conference Date | April 26-30, 1981 |
| Google Analytic Metrics | Metrics page |