Strains of infectious hematopoietic necrosis (IHN) virus may be identified by structural protein differences

Developments in Biological Standardization
By: , and 
Edited by: D.P. Anderson

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Abstract

The development of an effective vaccine to infectious hematopoietic necrosis virus (IHNV) in fish requires a knowledge of the virus serotypes in nature. At least two serotypes were found among three IHNV strains (12). Attempts in our laboratory to extend this study with additional virus strains by classical immunological techniques were unsatisfactory. Thus, we sought another method for comparing IHNV strains. We report here that different strains of IHNV can be distinguished by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the viron polypeptides. Major strain differences were noted in the molecular weights of the envelope glycoprotein, G, and the nucleocapsid protein, N.

The strains of IHNV were established from samples taken at several locations in California, Oregon, Washington, and Alaska. These strains were also characterized by growth rate at 15° and 18°C and average plaque size. Distinct differences in growth rate at the two temperatures were found for the California strains. The California strains were distinguished from the other strains by the production of minute plaques.

Additional publication details

Publication type Conference Paper
Publication Subtype Conference Paper
Title Strains of infectious hematopoietic necrosis (IHN) virus may be identified by structural protein differences
Series title Developments in Biological Standardization
ISBN 3-8055-3471-X
Volume 49
Year Published 1981
Language English
Publisher S. Karger
Publisher location Basel, Switzerland
Contributing office(s) Alaska Science Center, Western Fisheries Research Center
Description 13 p.
Larger Work Type Book
Larger Work Subtype Conference publication
Larger Work Title Fish biologics: Serodiagnostics and vaccines (Developments in biological standardization, volume 49)
First page 43
Last page 55
Conference Title International Symposium on Fish Biologics
Conference Location Leetown, WV
Conference Date April 26-30, 1981
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