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Sex identification of polar bears from blood and tissue samples

Canadian Journal of Zoology

By:
, , , and
https://doi.org/10.1139/z93-305

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Abstract

Polar bears (Ursus maritimus) can be adversely affected by hunting and other human perturbations because of low population densities and low reproduction rates. The sustainable take of adult females may be as low as 1.5% of the population. Females and accompanying young are most vulnerable to hunting, and hunters have not consistently reported the sex composition of the harvest, therefore a method to confirm the sexes of polar bears harvested in Alaska is needed. Evidence of the sex of harvested animals is often not available, but blood or other tissue samples often are. We extracted DNA from tissue and blood samples, and amplified segments of zinc finger (ZFX and ZFY) genes from both X and Y chromosomes with the polymerase chain reaction. Digestion of amplified portions of the X chromosome with the restriction enzyme HaeIII resulted in subdivision of the original amplified segment into four smaller fragments. Digestion with HaeIII did not subdivide the original segment amplified from the Y chromosome. The differing fragment sizes produced patterns in gel electrophoresis that distinguished samples from male and female bears 100% of the time. This technique is applicable to the investigation of many wildlife management and research questions.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Sex identification of polar bears from blood and tissue samples
Series title:
Canadian Journal of Zoology
DOI:
10.1139/z93-305
Volume:
71
Issue:
11
Year Published:
1993
Language:
English
Publisher:
NRC Research Press
Contributing office(s):
Alaska Science Center
Description:
4 p.
First page:
2174
Last page:
2177
Country:
United States
State:
Alaska