Geobacter bemidjiensis sp. nov. and Geobacter psychrophilus sp. nov., two novel Fe(III)-reducing subsurface isolates

International Journal of Systematic and Evolutionary Microbiology
By: , and 

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Abstract

Fe(III)-reducing isolates were recovered from two aquifers in which Fe(III) reduction is known to be important. Strain Bem was enriched from subsurface sediments collected in Bemidji, MN, USA, near a site where Fe(III) reduction is important in aromatic hydrocarbon degradation. Strains P11, P35 and P39 were isolated from the groundwater of an aquifer in Plymouth, MA, USA, in which Fe(III) reduction is important because of long-term inputs of acetate as a highway de-icing agent to the subsurface. All four isolates were Gram-negative, slightly curved rods that grew best in freshwater media. Strains P11, P35 and P39 exhibited motility via means of monotrichous flagella. Analysis of the 16S rRNA and genes indicated that all four strains are -proteobacteria and members of the cluster of the . Differences in phenotypic and phylogenetic characteristics indicated that the four isolates represent two novel species within the genus . All of the isolates coupled the oxidation of acetate to the reduction of Fe(III) [iron(III) citrate, amorphous iron(III) oxide, iron(III) pyrophosphate and iron(III) nitrilotriacetate]. All four strains utilized ethanol, lactate, malate, pyruvate and succinate as electron donors and malate and fumarate as electron acceptors. Strain Bem grew fastest at 30 °C, whereas strains P11, P35 and P39 grew equally well at 17, 22 and 30 °C. In addition, strains P11, P35 and P39 were capable of growth at 4 °C. The names sp. nov. (type strain Bem=ATCC BAA-1014=DSM 16622=JCM 12645) and sp. nov. (strains P11, P35 and P39; type strain P35=ATCC BAA-1013=DSM 16674=JCM 12644) are proposed.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Geobacter bemidjiensis sp. nov. and Geobacter psychrophilus sp. nov., two novel Fe(III)-reducing subsurface isolates
Series title International Journal of Systematic and Evolutionary Microbiology
DOI 10.1099/ijs.0.63417-0
Volume 55
Year Published 2005
Language English
Publisher Microbiology Society
Contributing office(s) Toxic Substances Hydrology Program
Description 8 p.
First page 1667
Last page 1674