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Development of twelve microsatellite loci in the red tree corals Primnoa resedaeformis and Primnoa pacifica

Conservation Genetics Resources

By:
https://doi.org/10.1007/s12686-015-0455-1

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Abstract

A suite of tetra-, penta-, and hexa-nucleotide microsatellite loci were developed from Roche 454 pyrosequencing data for the cold-water octocorals Primnoa resedaeformis and P. pacifica. Twelve of 98 primer sets tested consistently amplified in 30 P. resedaeformis samples from Baltimore Canyon (western North Atlantic Ocean) and in 24 P. pacifica samples (Shutter Ridge, eastern Gulf of Alaska). The loci displayed moderate levels of allelic diversity (average 7.5 alleles/locus) and heterozygosity (average 47 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and to distinguish species. These common species are long-lived (hundreds of years) and provide essential fish habitat (P. pacifica), yet populations are provided little protection from human activities. These loci will be used to determine regional patterns of population connectivity to inform effective marine spatial planning and ecosystem-based fisheries management.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Development of twelve microsatellite loci in the red tree corals Primnoa resedaeformis and Primnoa pacifica
Series title:
Conservation Genetics Resources
DOI:
10.1007/s12686-015-0455-1
Volume:
7
Issue:
3
Year Published:
2015
Language:
English
Publisher:
Springer
Contributing office(s):
Leetown Science Center
Description:
3 p.
First page:
763
Last page:
765