Fungal endophytes from seeds of invasive, non-native Phragmites australis and their potential role in germination and seedling growth

Plant and Soil
By: , and 

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Abstract

Background and aims

We characterized fungal endophytes of seeds of invasive, non-native Phragmites from three sites in the Great Lakes region to determine if fungal symbiosis could contribute to invasiveness through their effects on seed germination and seedling growth.

Methods

Field-collected seeds were surface sterilized and plated on agar to culture endophytes for ITS sequencing. Prevalence of specific endophytes from germinated and non-germinated seeds, and from seedlings, was compared.

Results

One-third of 740 seeds yielded endophyte isolates. Fifteen taxa were identified with Alternaria sp. representing 54% of all isolates followed by Phoma sp. (21%) and Penicillium corylophilum (12%). Overall germination of seeds producing an isolate (36%) was significantly higher than seeds not producing an isolate (20%). Penicillium in particular was strongly associated with increased germination of seeds from one site. Sixty-three isolates and 11 taxa were also obtained from 30 seedlings where Phoma, Penicillium and Alternaria respectively were most prevalent. There was a significant effect of isolating an endophyte from the seed on seedling growth.

Conclusions

These results suggest that many endophyte taxa are transmitted in seeds and can increase seed germination and seedling growth of invasive Phragmites. The role of fungal endophytes in host establishment, growth and invasiveness in nature requires further research.

Study Area

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Fungal endophytes from seeds of invasive, non-native Phragmites australis and their potential role in germination and seedling growth
Series title Plant and Soil
DOI 10.1007/s11104-017-3241-x
Volume 422
Issue 1-2
Year Published 2018
Language English
Publisher Springer
Contributing office(s) Great Lakes Science Center
Description 12 p.
First page 183
Last page 194
Country United States
State Michigan, Ohio