Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization
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Abstract
Butler’s Gartersnakes (BGS; Thamnophis butleri) are confined to open and semi-open canopy wetlands and adjacent uplands, habitats under threat of development in Wisconsin. To address issues of species identity and putative hybridization with congeneric snakes, a suite of 18 microsatellite loci capable of cross-species amplification of Plains Gartersnakes (T. radix) and Common Gartersnakes (T. sirtalis) was developed. All loci were polymorphic in BGS with mean number of alleles per locus of 16.11 (range = 3–41) and mean observed heterozygosity of 0.659 (range = 0.311–0.978). Loci amplified efficiently in the congeneric species with high levels of intra- and inter-specific variation. These loci will aid ongoing efforts to effectively identify and manage BGS in Wisconsin.
Publication type | Article |
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Publication Subtype | Journal Article |
Title | Novel microsatellite loci for studies of Thamnophis Gartersnake genetic identity and hybridization |
Series title | Conservation Genetics Resources |
DOI | 10.1007/s12686-011-9555-8 |
Volume | 4 |
Issue | 2 |
Year Published | 2012 |
Language | English |
Publisher | Springer |
Contributing office(s) | Coop Res Unit Leetown |
Description | 4 p. |
First page | 383 |
Last page | 386 |
Google Analytic Metrics | Metrics page |