A minimally invasive method for extraction of sturgeon oocytes

North American Journal of Aquaculture
By: , and 

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Abstract

Fishery biologists, hatchery personnel, and caviar fishers routinely extract oocytes from sturgeon (Acipenseridae) to determine the stage of maturation by checking egg quality. Typically, oocytes are removed either by inserting a catheter into the oviduct or by making an incision in the body cavity. Both methods can be time-consuming and stressful to the fish. We describe a device to collect mature oocytes from sturgeons quickly and effectively with minimal stress on the fish. The device is made by creating a needle from stainless steel tubing and connecting it to a syringe with polyvinyl chloride tubing. The device is filled with saline solution or water, the needle is inserted into the abdominal wall, and eggs are extracted from the fish. Using this device, an oocyte sample can be collected in less than 30 s. Such sampling leaves a minute wound that heals quickly and does not require suturing. The extractor device can easily be used in the field or hatchery, reduces fish handling time, and minimizes stress.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A minimally invasive method for extraction of sturgeon oocytes
Series title North American Journal of Aquaculture
DOI 10.1577/A09-006.1
Volume 72
Issue 2
Year Published 2010
Language English
Publisher American Fisheries Society
Contributing office(s) Columbia Environmental Research Center
Description 4 p.
First page 184
Last page 187