New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert
Conservation Genetics Resources
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Abstract
We isolated 16 novel microsatellite loci in two subspecies of endangered desert pronghorns (Antilocapra americana sonoriensis and Antilocapra americana peninsularis) using a shotgun pyrosequencing approach. All and 87.5 % of the loci were polymorphic within each subspecies, respectively. The mean number of alleles per locus was 4.86 (range 2–8) and 2.5 alleles per locus (range 1–4 alleles), and observed heterozygosity ranged from 0.13 to 0.78 (mean 0.48) and 0.00 to 0.61 (mean 0.31), respectively. We did not find significant linkage disequilibrium among loci pairs and only one locus deviated significantly from Hardy–Weinberg equilibrium in peninsularis.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-012-9749-8 |
| Volume | 5 |
| Issue | 1 |
| Year Published | 2013 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Coop Res Unit Seattle |
| Description | 3 p. |
| First page | 125 |
| Last page | 127 |
| Google Analytic Metrics | Metrics page |