New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert

Conservation Genetics Resources
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Abstract

We isolated 16 novel microsatellite loci in two subspecies of endangered desert pronghorns (Antilocapra americana sonoriensis and Antilocapra americana peninsularis) using a shotgun pyrosequencing approach. All and 87.5 % of the loci were polymorphic within each subspecies, respectively. The mean number of alleles per locus was 4.86 (range 2–8) and 2.5 alleles per locus (range 1–4 alleles), and observed heterozygosity ranged from 0.13 to 0.78 (mean 0.48) and 0.00 to 0.61 (mean 0.31), respectively. We did not find significant linkage disequilibrium among loci pairs and only one locus deviated significantly from Hardy–Weinberg equilibrium in peninsularis.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert
Series title Conservation Genetics Resources
DOI 10.1007/s12686-012-9749-8
Volume 5
Issue 1
Year Published 2013
Language English
Publisher Springer
Contributing office(s) Coop Res Unit Seattle
Description 3 p.
First page 125
Last page 127