Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads

Conservation Genetics Resources
By: , and 

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Abstract

We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7–19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy–Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads
Series title Conservation Genetics Resources
DOI 10.1007/s12686-013-0128-x
Volume 6
Issue 2
Year Published 2014
Language English
Publisher Springer
Contributing office(s) Coop Res Unit Seattle
Description 4 p.
First page 465
Last page 468