Strontium isotope ratios (⁸⁷Sr/⁸⁶Sr) in otoliths are a well-established tool to determine origins and movement patterns of fish. However, otolith extraction requires sacrificing fish, and when working with protected or endangered species, the use of nonlethal samples such as scales, spines, and fin rays is preferred. Unlike otoliths that are predominantly aragonite, these tissues are composed of biological apatite. Laser ablation multicollector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) analysis of biological apatite can induce significant interference on mass 87, causing inaccurate ⁸⁷Sr/⁸⁶Sr measurements. To quantify this interference, we applied LA-MC-ICP-MS to three marine samples (white seabass (Atractoscion nobilis) otolith; green sturgeon (Acipenser medirostris) pectoral fin ray; salmon shark (Lamna ditropis) tooth), and freshwater walleye (Sander vitreus) otoliths, scales, and spines). Instrument conditions that maximize signal intensity resulted in elevated ⁸⁷Sr/⁸⁶Sr isotope ratios in the bioapatite samples, related to a polyatomic interference (⁴⁰Ca³¹P¹⁶O, ⁴⁰Ar³¹P¹⁶O). Retuning instrument conditions to reduce oxide levels removed this interference, resulting in accurate ⁸⁷Sr/⁸⁶Sr ratios across all tissue samples. This method provides a novel, nonlethal alternative to otolith analysis to reconstruct fish life histories.