Separation of selenium species released from Se-exposed algae

By: , and 



We have assessed a fractionation scheme for selenium species that separates Se-containing amino acids and other organoselenium compounds in aqueous samples. We investigated the retention of standard solutions of selenate (Se+6), selenite (Se+4), and selenomethionine (Se−2) by fractionation media (Sephadex A-25 ion-exchange resin, copper-treated Chelex-100 ligand-exchange resin, and activated charcoal) and by several types of membrane filters. The fractionation method successfully isolated Se from the standard solutions into appropriate fractions for radiometric quantitation of 75Se. However, some filter media retained unacceptably large amounts of selenate and selenite. Mass balance microcosms were inoculated with green algae (Chlamydomonasreinhardtii) previously exposed to inorganic 75Se, and the fractionation scheme was used to examine the release of 75Se species into water and air. The results of the microcosm exposure indicate that seasonal blooms and crashes of phytoplankton populations may produce increased concentrations of organoselenium species.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Separation of selenium species released from Se-exposed algae
Series title Chemosphere
DOI 10.1016/0045-6535(94)90045-0
Volume 29
Issue 4
Year Published 1994
Language English
Publisher Elsevier
Contributing office(s) Columbia Environmental Research Center
Description 10 p.
First page 771
Last page 780
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