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A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation

BMC Research Notes

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https://doi.org/10.1186/s13104-018-3671-4

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Abstract

Objective

We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.

Results

Of the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4–337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1–9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
Series title:
BMC Research Notes
DOI:
10.1186/s13104-018-3671-4
Volume:
11
Year Published:
2018
Language:
English
Publisher:
BMC
Contributing office(s):
Wetland and Aquatic Research Center
Description:
Article 563; 5 p.
First page:
1
Last page:
5