A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation

BMC Research Notes
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Abstract

Objective

We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.

Results

Of the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4–337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1–9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
Series title BMC Research Notes
DOI 10.1186/s13104-018-3671-4
Volume 11
Year Published 2018
Language English
Publisher BMC
Contributing office(s) Wetland and Aquatic Research Center
Description Article 563; 5 p.
First page 1
Last page 5