A new tool for studying waterfowl immune and metabolic responses: Molecular level analysis using kinome profiling

Ecology and Evolution
By: , and 

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Abstract

Here, we describe the design of an Anas‐specific kinome peptide array that can be used to study the immunometabolic responses of mallard and American black duck to pathogens, contaminants, and environmental stress. The peptide arrays contain 2,642 unique phosphorylate‐able peptide sequences representing 1,900 proteins. These proteins cover a wide array of metabolic and immunological processes, and 758 Gene Ontology Biological processes are statistically significantly represented on the duck peptide array of those 164 contain the term “metabolic” and 25 “immune.” In addition, we conducted a comparison of mallard to American black duck at a genetic and proteomic level. Our results show a significant genomic and proteomic overlap between these two duck species, so that we have designed a cross‐reactive peptide array capable of studying both species. This is the first reported development of a wildlife species‐specific kinome peptide array.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A new tool for studying waterfowl immune and metabolic responses: Molecular level analysis using kinome profiling
Series title Ecology and Evolution
DOI 10.1002/ece3.4370
Volume 8
Issue 16
Year Published 2018
Language English
Publisher Wiley
Contributing office(s) Patuxent Wildlife Research Center
Description 10 p.
First page 8537
Last page 8546