Using redundant primer sets to detect multiple native Alaskan fish species from environmental DNA

Conservation Genetics Resources
By: , and 

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Abstract

Accurate and timely data regarding freshwater fish communities is important for informed decision-making by local, state, tribal, and federal land and resource managers; however, conducting traditional gear-based fish surveys can be an expensive and time-consuming process, particularly in remote areas, like those that characterize much of Alaska. To help address this challenge, we developed and tested five multi-species environmental DNA (eDNA) primer sets for the simultaneous detection of up to 37 target fish species in a single sample. Using these primer sets can reduce the cost and time needed to perform future studies of fish communities. Our results comparing multiple samples from multiple lakes and streams using multiple next-generation sequencing runs show the efficacy and reproducibility of these primers.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Using redundant primer sets to detect multiple native Alaskan fish species from environmental DNA
Series title Conservation Genetics Resources
DOI 10.1007/s12686-018-1071-7
Edition Online First
Year Published 2018
Language English
Publisher Springer
Contributing office(s) Alaska Science Center Biology WTEB