Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing
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Abstract
Mitochondrial genetic markers have been extensively used to study the phylogenetics and phylogeography of many birds, including seabirds of the order Procellariiformes. Evidence suggests that part of the mitochondrial genome of Procellariiformes, especially albatrosses, is duplicated, but no DNA fragment covering the entire duplication has been sequenced. We sequenced the complete mitochondrial genome of a non-albatross species of Procellariiformes, Puffinus lherminieri (Audubon’s shearwater) using the long-read MinION (ONT) technology. Two mitogenomes were assembled from the same individual, differing by 52 SNPs and in length. The shorter was 19 kb long while the longer was 21 kb, due to the presence of two identical copies of nad6, three tRNA, and two dissimilar copies of the control region (CR). Contrary to albatrosses, cobwas not duplicated. We further detected a complex repeated region of undetermined length between the CR and 12S. Long-read sequencing suggests heteroplasmy and a novel arrangement within the duplicated region, indicating a complex evolution of the mitogenome in Procellariiformes.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing |
| Series title | Mitochondrial DNA Part A |
| DOI | 10.1080/24701394.2018.1484116 |
| Volume | 30 |
| Issue | 2 |
| Year Published | 2019 |
| Language | English |
| Publisher | Taylor & Francis |
| Contributing office(s) | Patuxent Wildlife Research Center |
| Description | 8 p. |
| First page | 256 |
| Last page | 263 |
| Google Analytic Metrics | Metrics page |