Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the Aquareovirus A genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola |
| Series title | Diseases of Aquatic Organisms |
| DOI | 10.3354/dao03261 |
| Volume | 130 |
| Issue | 2 |
| Year Published | 2019 |
| Language | English |
| Publisher | Inter-Research |
| Contributing office(s) | Fort Collins Science Center, Leetown Science Center |
| Description | 14 p. |
| First page | 95 |
| Last page | 108 |
| Google Analytic Metrics | Metrics page |