The presence of antibiotic resistance genes in coastal soil and sediment samples from the eastern seaboard of the USA

Environmental Monitoring and Assessment
By: , and 

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Abstract

Infections from antibiotic resistant microorganisms are considered to be one of the greatest global public health challenges that result in huge annual economic losses. While genes that impart resistance to antibiotics (AbR) existed long before the discovery and use of antibiotics, anthropogenic uses of antibiotics in agriculture, domesticated animals, and humans are known to influence the prevalence of these genes in pathogenic microorganisms. It is critical to understand the role that natural and anthropogenic processes have on the occurrence and distribution of antibiotic resistance in microbial populations to minimize health risks associated with exposures. As part of this research, 15 antibiotic resistance genes were analyzed in coastal sediments and soils along the eastern seaboard of the USA using presence/absence quantitative and digital polymerase chain reaction assays. Samples (53 soil and 192 sediment samples including 54 replicates) were collected from a variety of coastal settings where human and wildlife exposure is likely. At least one of the antibiotic resistance genes was detected in 76.4% of the samples. Samples that contained at least five or more antibiotic resistance genes (5.7%) where typically hydrologically down gradient of watersheds influenced by combined sewer outfalls (CSO). The most frequently detected antibiotic resistance target genes were found in 33.2%, 34.4%, and 42.2% of samples (target genes blaSHVtetO, and aadA2, respectively). These data provide unique insight into potential exposure of AbR genes over a large geographical region of the eastern seaboard of the USA.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title The presence of antibiotic resistance genes in coastal soil and sediment samples from the eastern seaboard of the USA
Series title Environmental Monitoring and Assessment
DOI 10.1007/s10661-019-7426-z
Volume 19
Issue Suppl 2
Year Published 2019
Language English
Publisher Springer International Publishing
Contributing office(s) St. Petersburg Coastal and Marine Science Center, Crustal Geophysics and Geochemistry Science Center, Central Mineral and Environmental Resources Science Center, New Jersey Water Science Center, New York Water Science Center, Office of the AD Energy and Minerals, and Environmental Health, Toxic Substances Hydrology Program, Leetown Science Center, Kansas Water Science Center, Eastern Geographic Science Center, Utah Water Science Center
Description 300, 17 p.
Country United States
State Connecticut, Delaware, District of Columbia, Maine, Maryland, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, South Carolina, Virginia