Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America

Conservation Genetics Resources
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Abstract

We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America
Series title Conservation Genetics Resources
DOI 10.1007/s12686-019-01118-7
Edition Online First
Year Published 2019
Language English
Publisher Springer
Contributing office(s) Western Fisheries Research Center
Country United States
Other Geospatial Pacific Northwest