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Characterization of immunoglobulin light chain utilization and variable family diversity in rainbow trout

Developmental and Comparative Immunology
By: , , , , and 
https://doi.org/10.1016/j.dci.2019.103566

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Abstract

This study characterizes immunoglobulin light chain (IgL) expression and variable family usage in rainbow trout. IgL transcripts were generated by 5’ RACE from both immune and TNP-KLH immunized fish. Phylogenetic analysis revealed that the IgL variable regions clustered into seven different families: three kappa families (two newly described in this study), three sigma families, and a single lambda family. IgL1 and IgL3 transcripts expressing identical variable regions were identified and genomic analysis revealed that the two isotypes are co-localized on chromosomes 7, 15, 18, and 21 allowing for potential rearrangement between clusters. Fish were immunized with TNP-KLH (n = 5) and percent expression of IgL1, IgL2, IgL3, and IgL4 measured by qRT-PCR from immune tissues and magnetically sorted TNP-specific lymphocyte populations. In all samples IgL1 constituted 80–95% of the transcripts. The percentage of anti-TNP specific IgL1 transcripts was measured in naïve, unsorted, and TNP-specific cell populations of TNP-KLH fish (n = 3) and found to be significantly higher in the TNP positive cell population (21%) compared to the naïve population (1%; p = 0.02) suggesting that there is a selection of TNP specific IgL sequences.
Publication type Article
Publication Subtype Journal Article
Title Characterization of immunoglobulin light chain utilization and variable family diversity in rainbow trout
Series title Developmental and Comparative Immunology
DOI 10.1016/j.dci.2019.103566
Volume 104
Year Published 2019
Language English
Publisher Elsevier
Contributing office(s) Western Fisheries Research Center
Description 103566, 11 p.
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