Streamflow estimation methods that transfer information from an index gage to an ungaged site are commonly used; however, uncertainty in daily streamflow estimates are often not adequately quantified. In this study, daily streamflow was simulated at 1,331 validation streamgages across the continental United States using four transfer-based streamflow estimation methods. Empirical 95 percent uncertainty intervals were computed for estimated daily streamflows. Uncertainty intervals were evaluated for reliability, sharpness, and overall ability to accurately quantify the uncertainty inherent in the estimated daily streamflow. Uncertainty intervals performed reliably in the Eastern U.S. and Pacific Northwest regions of the country, containing a median of 96 and 99 percent of the observed values respectively. Uncertainty intervals were less reliable in the Great Plains and arid Southwest regions, where uncertainty intervals contained a median of 83 and 94 percent of the observed streamflows respectively. Uncertainty interval performance was correlated with gage density and hydrologic similarity near the validation site, as well as the aridity and base-flow indices at the site.