Capture of environmental DNA (eDNA) from water samples by flocculation

JOVE Journal Of Visualized Experiments
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Abstract

The analysis of environmental DNA (eDNA) has become a widely used approach to problem solving in species management. The detection of cryptic species including invasive and (or) species at risk is the goal, typically accomplished by testing water and sediment for the presence of characteristic DNA signatures. Reliable and efficient procedures for the capture of eDNA are required, especially those that can be performed easily in the field by personnel with limited training and citizen scientists. The capture of eDNA using membrane filtration is widely used currently. This approach has inherent issues that include the choice of filter material and porosity, filter fouling, and time required on site for the process to be performed. Flocculation offers an alternative that can be easily implemented and applied to sampling regimes that strive to cover broad territories in limited time.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Capture of environmental DNA (eDNA) from water samples by flocculation
Series title JOVE Journal Of Visualized Experiments
DOI 10.3791/60967
Volume 159
Year Published 2020
Language English
Publisher JOVE
Contributing office(s) Leetown Science Center
Description e60967
Online Only (Y/N) Y
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