During 2012-2014, we evaluated macroinvertebrate communities in streams draining the non-connected stormwater management region (Municipal Separate Storm Sewer System, or MS4) within the Kansas City metropolitan area utilizing the Missouri bioassessment protocols. Trends in aquatic life impairment status based on Missouri's Macroinvertebrate Stream Condition Index (MSCI), as well as richness and abundance of EPT indicator metrics (Ephemeroptera, Plecoptera, Trichoptera), were compared between rural control sites and both transitional and urban stream sites representing varying stages of land use conversion. As compared to non-urban control sites, EPT taxa richness was significantly lower at MS4 urban sites during all three years (p = 0.007 – 0.013) and MS4 transitional sites during one of three years (p=0.48). EPT abundance (%) was significantly lower at MS4 urban sites during all years (p = 0.008 – 0.013) and MS4 transitional sites during one of three years (p=0.34). Mean EPT abundances ranged between 0.6% - 10.3% at urban MS4 sites, and always exceeded 18% at control sites. Both EPT richness and abundance were lower at the MS4 control site but means for EPT and other core metrics at this site were most often similar to non-urban control sites based on analysis of variance (ANOVA). MS4 transitional sites with active development in their watersheds were partially-supporting in their impairment status, and EPT metrics had lower means and generally more variability than control sites. Temporal trends indicate non-urban control and MS4 control sites consistently meet fully-supporting impairment status based on overall MSCI scores, but no study sites currently meet regional expectations (as defined by state reference streams) for either of the EPT metrics. Results indicate that Missouri and Kansas biocriteria for both EPT metrics are not consistently being met at any stream sites in the Kansas City metro area, including fully-supporting control sites and MS4 streams that receive stormwater runoff in watersheds with urban development that is well-established or currently transitioning to urban or suburban land uses.