In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
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Abstract
Publication type | Article |
---|---|
Publication Subtype | Journal Article |
Title | Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques |
Series title | Archives of Virology |
DOI | 10.1007/s00705-020-04737-w |
Volume | 165 |
Year Published | 2020 |
Contributing office(s) | National Wildlife Health Center |
Description | 5 p. |
First page | 2373 |
Last page | 2377 |
Google Analytic Metrics | Metrics page |