Laboratory maintenance and culture of Pseudogymnoascus destructans, the fungus that causes bat white-nose syndrome

Current Protocols
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Abstract

Pseudogymnoascus destructans is a fungal pathogen that causes white‐nose syndrome, an emerging and fatal disease of North American bats that has led to unprecedented population declines. As a psychrophile, P. destructans is adapted to infect bats during winter hibernation, when host metabolic activity and core body temperature are greatly reduced. The ability to maintain and cultivate isolates of P. destructans in the laboratory is necessary for conducting research with this fungus. This article describes protocols for culturing P. destructans from bat wing skin and soil, for cryopreserving the fungus, and for preparing liquid suspensions for laboratory experimentation. 

Publication type Article
Publication Subtype Journal Article
Title Laboratory maintenance and culture of Pseudogymnoascus destructans, the fungus that causes bat white-nose syndrome
Series title Current Protocols
DOI 10.1002/cpz1.23
Volume 1
Issue 1
Year Published 2021
Language English
Publisher Wiley
Contributing office(s) National Wildlife Health Center
Description e23, 16 p.
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