Sex- and developmental stage-related differences in the hepatic transcriptome of Japanese quail (Coturnix japonica) exposed to 17β-Trenbolone
Endocrine disrupting chemicals (EDCs) can cause transcriptomic changes that may disrupt biological processes associated with reproductive function including metabolism, transport, and cell growth. We investigated effects from in ovo and dietary exposure to 17β-trenbolone (17βT; at 0, 1 and 10 ppm) on the Japanese quail (Coturnix japonica) hepatic transcriptome. Our objectives were to identify differentially expressed hepatic genes, assess perturbations of biological pathways, and examine sex- and developmental stage-related differences. The number of significantly differentially expressed genes was higher in embryos than in adults. Male embryos exhibited greater differential gene expression than female embryos, whereas in adults, males and females exhibited similar numbers of differentially expressed genes (>2-fold). Vitellogenin and apovitellenin-1 were upregulated in male adults exposed to 10 ppm 17βT and these birds also exhibited indications of immunomodulation. Functional grouping of differentially expressed genes identified processes including metabolism and transport of biomolecules, enzyme activity and extracellular matrix interactions. Pathway enrichment analyses identified peroxisome proliferator-activated receptor pathway, cardiac muscle contraction, gluconeogenesis, growth factor signaling, focal adhesion, and bile acid biosynthesis as perturbed. One of the primary uses of 17βT is that of a growth promoter and these results identify effects on mechanistic pathways related to steroidogenesis, cell proliferation, differentiation, and growth, and metabolism of lipids and proteins.
|Publication Subtype||Journal Article|
|Title||Sex- and developmental stage-related differences in the hepatic transcriptome of Japanese quail (Coturnix japonica) exposed to 17β-Trenbolone|
|Series title||Environmental Toxicology and Chemistry|
|Publisher||Society of Environmental Toxicology and Chemistry|
|Contributing office(s)||Eastern Ecological Science Center|
|Google Analytic Metrics||Metrics page|