Beginning in February of 2020, researchers and staff of the United States Geological Survey (USGS), Bat Conservation International (BCI), Virginia Polytechnic Institute and State University, and Montana State University associated with the North American Bat Monitoring Program (NABat) collaborated with the United States Fish and Wildlife Service (USFWS) to provide technical assistance in support of the USFWS Three Bat Species Status Assessments (SSA) including the little brown bat (MYLU, Myotis lucifugus), northern long-eared bat (MYSE, Myotis septentrionalis), and tricolored bat (PESU, Perimyotis subflavus). Analytical support for the SSA was not intended to provide interpretive results, which should therefore be considered beyond the scope of this report. Technical assistance for the SSA included facilitating the USFWS data call by educating and working directly with data contributors to manage, submit, and archive a variety of bat monitoring data in the online NABat database1 using standardized data submission templates accessible through the upload features on the NABat website. Most data collected through the USFWS data call for the SSA were submitted and stored in the NABat database. These represent the efforts of hundreds of partnering organizations across more than 200 individual NABat Partner Portal Projects. A few organizations contributed data for use in these analyses that were not submitted to the NABat database. Records submitted in response to the USFWS data call included bat capture, stationary and mobile acoustic, and internal roost count data (winter and summer). Data collated in the NABat database and used for the analyses described herein are documented (NABat 2020a, NABat 2020b, NABat 2021) and available through the NABat third party request feature.