The DLR Earth Sensing Imaging Spectrometer (DESIS) is the first hyperspectral imaging spectrometer installed in the Multi-User System for Earth Sensing (MUSES) on the International Space Station (ISS) for acquiring routine science grade images from orbit. It was launched on 29 June 2018 and integrated into MUSES. DESIS measures energy in the spectral range of 400 to 1000 nm with high spatial and spectral resolution: 30 m and 2.55 nm, respectively. DESIS data should be sufficiently quantitative and accurate to use it for different applications and research. This work performs a radiometric evaluation of DESIS Level 1 product (Top of Atmosphere (TOA) reflectance) by comparing it with coincident Radiometric Calibration Network (RadCalNet) measurements at Railroad Valley Playa (RVUS), Gobabeb (GONA), and La Crau (LCFR). RVUS, GONA, and LCFR offer 4, 15, and 5 coincident datasets between DESIS and RadCalNet measurements, respectively. The results show an agreement between DESIS and RadCalNet TOA reflectance within ~5% for most spectral regions. However, there is an additional ~5% disagreement across the wavelengths affected by water vapor absorption and atmospheric scattering. Among the three RadCalNet sites, RVUS and GONA show a similar measurement disagreement with DESIS of ~5%, while LCFR differs by ~10%. Agreement between DESIS and RadCalNet measurements is variable across all three sites, likely due to surface type differences. DESIS and RadCalNet agreement show a precision of ~2.5%, 4%, and 7% at RVUS, GONA, and LCFR, respectively. RVUS and GONA, which have a similar surface type, sand, have a similar level of radiometric accuracy and precision, whereas LCFR, which consists of sparse vegetation, has lower accuracy and precision. The observed precision of DESIS Level 1 products from all the sites, especially LCFR, can be improved with a better Bidirectional Reflection Distribution Function (BRDF) characterization of the RadCalNet sites.