The information in this report was compiled to aid in the application and interpretation of historical water-quality data collected as part of the U.S. Geological Survey Hydrologic Benchmark Network program, which was established in 1963 to provide long-term measurements of streamflow and water quality in areas that are minimally affected by human activities. This report describes the environmental characteristics and water quality at 16 benchmark basins in the Eastern United States. In most of the basins, stream-water chemistry seems to be controlled by the interaction of acidic precipitation with the underlying soils and bedrock. Land use had a minimal effect on stream-water chemistry at most of the gaging stations.