The Brookian megasequence in the National Petroleum Reserve in Alaska (NPRA) includes bottomset and clinoform seismic facies of the Torok Formation (mostly Albian age) and generally coeval, topset seismic facies of the uppermost Torok Formation and the Nanushuk Group. These strata are part of a composite total petroleum system involving hydrocarbons expelled from three stratigraphic intervals of source rocks, the Lower Cretaceous gamma-ray zone (GRZ), the Lower Jurassic Kingak Shale, and the Triassic Shublik Formation. The potential for undiscovered oil and gas resources in the Brookian megasequence in NPRA was assessed by defining five plays (assessment units), one in the topset seismic facies and four in the bottomset-clinoform seismic facies. The Brookian Topset Play is estimated to contain between 60 (95-percent probability) and 465 (5-percent probability) million barrels of technically recoverable oil, with a mean (expected value) of 239 million barrels. The Brookian Topset Play is estimated to contain between 0 (95-percent probability) and 679 (5-percent probability) billion cubic feet of technically recoverable, nonassociated natural gas, with a mean (expected value) of 192 billion cubic feet. The Brookian Clinoform North Play, which extends across northern NPRA, is estimated to contain between 538 (95-percent probability) and 2,257 (5-percent probability) million barrels of technically recoverable oil, with a mean (expected value) of 1,306 million barrels. The Brookian Clinoform North Play is estimated to contain between 0 (95-percent probability) and 1,969 (5-percent probability) billion cubic feet of technically recoverable, nonassociated natural gas, with a mean (expected value) of 674 billion cubic feet. The Brookian Clinoform Central Play, which extends across central NPRA, is estimated to contain between 299 (95-percent probability) and 1,849 (5-percent probability) million barrels of technically recoverable oil, with a mean (expected value) of 973 million barrels. The Brookian Clinoform Central Play is estimated to contain between 1,806 (95-percent probability) and 10,076 (5-percent probability) billion cubic feet of technically recoverable, nonassociated natural gas, with a mean (expected value) of 5,405 billion cubic feet. The Brookian Clinoform South-Shallow Play is estimated to contain between 0 (95-percent probability) and 1,254 (5-percent probability) million barrels of technically recoverable oil, with a mean (expected value) of 508 million barrels. The Brookian Clinoform South-Shallow Play is estimated to contain between 0 (95-percent probability) and 5,809 (5-percent probability) billion cubic feet of technically recoverable, nonassociated natural gas, with a mean (expected value) of 2,405 billion cubic feet. The Brookian Clinoform South-Deep Play is estimated to contain between 0 (95-percent probability) and 8,796 (5-percent probability) billion cubic feet of technically recoverable, nonassociated natural gas, with a mean (expected value) of 3,788 billion cubic feet. No technically recoverable oil is assessed in the Brookian Clinoform South-Deep Play, as it lies at depths that are entirely in the gas window. Among the Brookian stratigraphic plays in NPRA, the Brookian Clinoform North Play and the Brookian Clinoform Central Play are most likely to be objectives of exploration activity in the near-term future because they are estimated to contain multiple oil accumulations larger than 128 million barrels technically recoverable oil, and because some of those accumulations may occur near existing infrastructure in the eastern parts of the plays. The other Brookian stratigraphic plays are not likely to be the focus of exploration activity because they are estimated to contain maximum accumulation sizes that are smaller, but they may be an objective of satellite exploration if infrastructure is extended into the play areas. The total volumes of natural gas estimated to occur in B