Those ostracode species described by Ulrich and Bassler (1904) and Malkin (1953) from the Chesapeake Group of early Miocene to early Pleistocene age are treated systematically. Lectotypes are designated for all species which have syntypic suites and the lectotypes, holotypes, or hypotypes for all but two species are illustrated. A new genus Otikocythere is proposed, with Cythere punctistriata Ulrich and Bassler 1904 as the type species. A new species, Hulingsina calvertensis, is also proposed. All of the species are placed in an updated biostratigraphic framework.