Overview and Methodology for a Study To Identify Fecal Contamination Sources Using Microbial Source Tracking in Seven Embayments on Long Island, New York
Between June 2018 and July 2019, the U.S. Geological Survey collaborated with the New York State Department of Environmental Conservation to analyze water quality in seven embayments on Long Island, New York, for a study to examine fecal contamination using microbial source tracking. This report documents the approach, methodology, and quality-assurance data used in the study. All samples and field data were collected in accordance with U.S. Geological Survey National Field Manual procedures. Samples were analyzed for host-specific deoxyribonucleic acid (DNA) markers, fecal coliform bacteria, inorganic and total organic nitrogen, and stable isotopes of nitrate and ammonium.
Samples for quality control were collected for microbiological analyses at a rate of 1 per 20 environmental samples. A total of 14 blank and 15 replicate samples were collected for DNA markers, 52 sequential field replicates were analyzed by the Public Environmental Health Laboratory of the Suffolk County Department of Health Services and the New York State Department of Conservation Marine Laboratory for fecal coliform, and 7 blank and 7 replicate samples were collected to be analyzed for nutrients. Results from quality-control samples collected throughout the course of the study confirmed that sampling procedures were adequate and did not disqualify any data from analysis.
Tagliaferri, T.N., Fisher, S.C., Kephart, C.M., Cheung, N., Reed, A.P., and Welk, R.J., 2021, Overview and methodology for a study to identify fecal contamination sources using microbial source tracking in seven embayments on Long Island, New York: U.S. Geological Survey Scientific Investigations Report 2021–5033, 8 p., https://doi.org/10.3133/sir20215033.
ISSN: 2328-0328 (online)
|Publication Subtype||USGS Numbered Series|
|Title||Overview and methodology for a study to identify fecal contamination sources using microbial source tracking in seven embayments on Long Island, New York|
|Series title||Scientific Investigations Report|
|Publisher||U.S. Geological Survey|
|Publisher location||Reston, VA|
|Contributing office(s)||New York Water Science Center, Ohio-Kentucky-Indiana Water Science Center|
|Description||iv, 8 p.|
|Other Geospatial||Long Island|
|Online Only (Y/N)||Y|
|Google Analytic Metrics||Metrics page|