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Extraction and analysis of adenosine triphosphate from aquatic environments

Water-Resources Investigations Report 81-5

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Abstract

A variety of adenosine triphosphate (ATP) extraction procedures have been investigated for their applicability to samples from aquatic environments. The cold sulfuric-oxalic acid procedure was best suited to samples consisting of water, periphyton, and sediments. Due to cation and fulvic acid interferences, a spike with a known quantity of ATP was necessary to estimate losses when sediments were extracted. Variable colonization densities for periphyton required that several replicates be extracted to characterize acdurately the periphyton community. Extracted samples were stable at room temperature for one to five hours, depending on the ATP concentration, if the pH was below 2. Neutralized samples which were quick frozen and stored at -30°C were stable for months.

Additional publication details

Publication type:
Report
Publication Subtype:
USGS Numbered Series
Title:
Extraction and analysis of adenosine triphosphate from aquatic environments
Series title:
Water-Resources Investigations Report
Series number:
81-5
Year Published:
1981
Language:
English
Publisher:
U.S. Geological Survey
Description:
iv, 28 p.