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Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology

Applied and Environmental Microbiology

By:
,
DOI: 10.1128/AEM.01303-08

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Abstract

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem. Copyright ?? 2008, American Society for Microbiology. All Rights Reserved.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology
Series title:
Applied and Environmental Microbiology
DOI:
10.1128/AEM.01303-08
Volume
74
Issue:
24
Year Published:
2008
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
7828
Last page:
7831
Number of Pages:
4