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Enhancement of anti-Aeromonas salmonicida activity in Atlantic salmon (Salmo salar) macrophages by a mannose-binding lectin

Comparative Biochemistry and Physiology, Part C: Pharmacology, Toxicology and Endocrinology

By:
, , , , and
https://doi.org/10.1016/S0742-8413(99)00009-2

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Abstract

We investigated the effects of a calcium-dependent mannose-binding lectin isolated from the serum of Atlantic salmon on Aeromonassalmonicida viability and the anti-A. salmonicida activity of Atlantic salmon macrophages. In the absence of other factors, binding of this lectin at concentrations of 0.8, 4.0 and 20.0 ng ml−1 to virulent A. salmonicida failed to significantly reduce (P>0.05) cell viability. However, binding of the lectin to A. salmonicida did result in significant (P≤0.05) dose-dependent increases in phagocytosis, and bactericidal activity. Significant increases (P≤0.05) were also observed in phagocyte respiratory burst activity within the lectin concentration range of 4.0–20.0 ng ml−1 but the stimulation was not dose dependent at these lectin concentrations. At the lowest lectin concentration tested (0.32 ng ml−1), a significant decrease (P≤0.05) in respiratory burst was observed. The structure and activity of this lectin are similar to that of mammalian mannose-binding lectins, which are known to play a pivotal role in innate immunity. The presence of this lectin may be an important defense mechanism against Gram-negative bacteria such as A. salmonicida.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Enhancement of anti-Aeromonas salmonicida activity in Atlantic salmon (Salmo salar) macrophages by a mannose-binding lectin
Series title:
Comparative Biochemistry and Physiology, Part C: Pharmacology, Toxicology and Endocrinology
DOI:
10.1016/S0742-8413(99)00009-2
Volume:
123
Issue:
1
Year Published:
1999
Language:
English
Publisher:
Elsevier
Contributing office(s):
Leetown Science Center
Description:
7 p.
First page:
53
Last page:
59